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Williams’ E Medium – Max

$36.26$715.78

Williams’ E Medium – Max contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life while significantly improving cell viability and growth. We manufacture several types of Williams’ E Medium based on your requirements.

Description

Williams’ E Medium – Max

Background

Williams’ E Medium – Max is a commonly used media for Mammalian cell culture.

Advantages

The advantages of Williams’ E Medium – Max over Williams’ E Medium are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

Williams’ E Medium – Max contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of William’s E Medium based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

Williams’ E Medium – Max contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

For complete formulation click here: Williams’ E Medium-MAX Formulation

References

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

William’s E -MAX Standard Formulation, William’s E -MAX w/o Phenol Red, William’s E -MAX w/o Sodium Bicarbonate, William’s E -MAX w/o Sodium Pyruvate, William’s E -MAX w/o Phenol Red, w/o Sodium Bicarbonate, William’s E -MAX Low Sodium Bicarbonate (1.1 g/L), William’s E -MAX 15 mM HEPES (3.6 g/L), William’s E -MAX 25 mM HEPES (5.9 g/L), William’s E -MAX 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), William’s E -MAX 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.1 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

Quality Control

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Formulation

William E-MAX Formulation

SDS

SDS

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