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- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
PurMa™ Iscove’s Modified Dulbecco’s Medium (IMDM)
A high-buffer, high-glucose basal medium optimized for high-density, fast-growing cell lines.
- Suited to demanding lines such as Jurkat, HEK, COS-7, and macrophages
- High buffering capacity and high glucose for fast growth
Description
Product Description
PurMa™ Iscove’s Modified Dulbecco’s Medium (IMDM) is a robust basal medium designed for rapidly proliferating and high‑density mammalian cell cultures. PurMa™ IMDM delivers a stable pH environment with elevated bicarbonate and HEPES buffering, making it well suited for demanding cultures and procedures that require consistent pH control.
Performance and applications of PurMa™ Iscove’s Modified Dulbecco’s Medium (IMDM) support high‑density growth and frequent passaging. It is commonly used for suspension and adherent lines that require enriched nutrients and stable buffering, including Jurkat, HEK, COS‑7, and various macrophage and immune cell models. Because IMDM cell culture media replaces ferric nitrate with potassium nitrate, it lacks free iron, which can benefit adhesive cell cultures sensitive to iron‑mediated oxidation.
Key formulation highlights
| Component | Typical concentration |
|---|---|
| NaHCO₃ (Sodium bicarbonate) | 3.02 g/L |
| HEPES | 5.9 g/L |
| Glucose (high) | 4.5 g/L |
| L‑glutamine | 0.584 g/L |
| Sodium pyruvate | 110 mg/L (1.0 mM) |
| Phenol red | 15 mg/L |
Benefits of PurMa™ Iscove’s Modified Dulbecco’s Medium (IMDM)
- Consistent performance: manufactured under strict QC with Certificates of Analysis for each lot.
- Enhanced stability: buffering and nutrient profile reduce culture stress during incubator disturbances.
- Versatile: suitable for both suspension and adherent work at high cell densities.
- Application ready: ideal for transfection protocols, hybridoma culture, immune cell expansion, and production workflows.
Handling & storage Store unopened IMDM at 2–8 °C and protect from light. Warm to 37 °C before use and supplement per your protocol (e.g., 5–10% FBS or defined supplements). Avoid repeated freeze–thaw cycles. Validate performance with your cell lines before large‑scale use.
Documentation & support Complete Iscove’s Modified Dulbecco’s Medium (IMDM) formulation and Certificate of Analysis (COA) are available on our website. PurMa technical support can assist with media optimization, supplementation strategies, and protocol adaptation.
Complete formulation is available on our website as well as here : IMDM Formulation
FAQs
Q1: What is IMDM iscoves modified dulbeccos medium ) used for?
A1: IMDM (Iscove’s Modified Dulbecco’s Medium) is used for high‑density, rapidly proliferating mammalian cell cultures, including Jurkat, HEK, COS‑7, macrophages, and hybridomas.
Q2: How does IMDM differ from DMEM?
A2: IMDM has an enriched vitamin and amino acid profile, higher buffering (NaHCO3 + HEPES), and uses potassium nitrate instead of ferric nitrate, improving performance for some adhesive cells.
Q3: Does PurMa IMDM contain L‑glutamine?
A3: Yes. PurMa IMDM contains 0.584 g/L L‑glutamine; handle and store per lab SOPs and supplement when required.
Q4: How should I store and prepare IMDM?
A4: Store unopened at 2–8 °C, protect from light, and warm to 37 °C before use. Supplement with serum or defined additives per your protocol and avoid repeated freeze–thaw cycles.
Q5: Is IMDM cell culture suited for serum‑free form of medium?
A5: IMDM can be used in serum‑free or defined systems with appropriate supplementation of growth factors, hormones, or serum replacements depending on your cell type.
References
- Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Learn more: What Is IMDM?
Additional information
| Condition | IMDM Standard Formulation, IMDM Standard Formulation, ATCC Formulation, IMDM w/o Glutamine, IMDM w/o Phenol Red, IMDM w/o Sodium Bicarbonate, IMDM w/o Sodium Pyruvate, IMDM w/o Glucose, IMDM w/o HEPES, IMDM w/o Glutamine, w/o Phenol Red, IMDM w/o Phenol Red, w/o Sodium Bicarbonate, IMDM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, IMDM w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, IMDM Low Glucose (1.5 g/L), IMDM 15 mM HEPES (3.6 g/L), IMDM 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), IMDM w/o Sodium Pyruvate, w/o Phenol Red, IMDM w/o Sodium Pyruvate, w/o HEPES, IMDM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), IMDM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), IMDM w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), IMDM w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
Frequently asked questions
How should I store PurMa™ Iscove’s Modified Dulbecco’s Medium (IMDM)?
Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.
What is the shelf life once opened?
Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.
Is it sterility and endotoxin tested?
Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.
Do I need to add L-glutamine?
It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.
Can I order this in bulk or as a custom formulation?
Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.
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